In the current study, the focus was placed on the characterization of gut structure changes in mouse models for Autism spectrum disorder and chronic obstructive pulmonary disease. Autism spectrum disorder (ASD) is defined as a developmental condition affecting behavior and communication. The disease implies a variation of symptoms and can depend from one patient to another (National Institute of Mental Health). Chronic obstructive pulmonary disease (COPD) is a lung disease associated with difficulties with breathing and poor airflow. In the study, histopathology tests were conducted, with villus height being greater in HIM compared to WT within the Shank3 KO mutation on mouse colon due to the association with the mutations of these genes. Villus height was shown to increase for the deletion of Shank3 deletion (663.2 ± 91 µm) compared to WT (537 ± 34.5 µm) with Shank3 KO villus height 571.7 ± -125.9 µm.z. Villus width in Shank3 mutant mice did not show significant differences, and crypt depth showed no differences in the same group also.
The test to detect Hu, Tuj-1, and Iba1 showed that strong immunoreactivity was present for the first two antibodies related to ASD and COPD presence. Quantitative measurements reveled Iba1’s higher density in Shank3 (n=6) compared to WT (n=6). In terms of mucus thickness, the blue stating of mid colon samples of Cigarette smoke (CS) (n=8) and Sham (n=7) mice. The thickness revealed to was larger in Sham compared to CS. While the findings show that the histology of immune cells in the samples of mice showed the impact of the mutations on the prevalence of ASD and COPD, future research on this topic is necessary to reveal additional details.
National Institute of Mental Health. “Autism Spectrum Disorder.” Nimh Nih. Web.